G7153
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G7153
Sigma
Guanidine hydrochloride
SigmaUltra, >99.5% (titration)
| Synonym: | Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride |
| CAS Number: | 50-01-1 |
| Linear Formula: | NH2C(=NH)NH2 · HCl |
| Molecular Weight: | 95.53 |
| Beilstein Registry Number: | 3591990 |
| EC Number: | 200-002-3 |
| MDL number: | MFCD00013026 |
| PubChem Substance ID: | 24895295 |
Description
| Application | Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. |
Properties
| grade | SigmaUltra |
| assay | >99.5% (titration) |
| total impurities | Insoluble matter, passes filter test |
| ign. residue | <0.05% |
| loss | <0.05% loss on drying, HV, 20°C |
| pH | 4.5-6.0 (20 °C, 6 M in H2O) |
| EQP level | Elite |
| mp | 180-185 °C(lit.) |
| solubility | H2O: 6 M at 20 °C, clear, colorless |
| anion traces | sulfate (SO42-): <0.005% |
| cation traces | Al: <0.0005% |
| As: <0.0001% | |
| Ba: <0.0005% | |
| Bi: <0.0005% | |
| Ca: <0.001% | |
| Cd: <0.0005% | |
| Co: <0.0005% | |
| Cr: <0.0005% | |
| Cu: <0.0005% | |
| Fe: <0.0005% | |
| K: <0.005% | |
| Li: <0.0005% | |
| Mg: <0.0005% | |
| Mn: <0.0005% | |
| Mo: <0.0005% | |
| Na: <0.005% | |
| Ni: <0.0005% | |
| Pb: <0.0005% | |
| Sr: <0.0005% | |
| Zn: <0.0005% | |
| abs. | A |
| A |
Safety
References
| reference | Mukhopadhyay, A., Inclusion bodies and purification of proteins in biologically active forms. Adv. Biochem. Eng. Biotechnol. 56, 61-109., (1997) Abstract |
| Levine, A.D., et al., High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia coli. J. Biol. Chem. 270, 7445-7452, (1995) | |
| Rudloph, R. and Lilie, H., In vitro folding of inclusion body proteins. FASEB J. 10, 49-56, (1996) Abstract | |
| Naglak, T.J. and Wang, H.Y., Recovery of a foreign protein from the periplasm of Escherichia coli by chemical permeabilization. Enzyme Microb. Technol. 12, 603, (1990) | |
| Marston, F.A.O. and Hartley, D.L., Solubilization of protein aggregates. Meth. Enzymol. 182, 264-276, (1990) | |
| Merck | Merck 13,4578 |
| Beilstein | Beil. 3,IV,150 |
| Aldrich MSDS 1, 998:C / Corp MSDS 1 (1), 1803:D / FT-IR 1 (1), 818:A / FT-IR 2 (1), 1388:B / IR-Spectra (3), 485:D / IR-Spectra (2), 429:D / RegBook 1 (1), 961:K / Sax 6, 1488 / Sigma FT-IR 1 (2), 597:C / Sigma FT-IR 1 (2), 597:D / Structure Index 1, 149:C:7 |
